The 35-year-old Gatlin, twice banned for doping, came through almost unnoticed in lane seven in 9.92 seconds, with Coleman's 9.94 holding off the greatest sprinter of all time.
Despite struggling for fitness and form in his valedictory season, Bolt had still been favourite to secure his 20th global gold.
It was supposed to be Gatlin's year in 2015, when the American went to the World Championships in Beijing on the back of a 28-race unbeaten run.
Bolt produced his great miracle to beat him that night in the Bird's Nest, but in the stadium where he won 100m Olympic gold in 2012, he could not provide the perfect ending to a perfect career.
Despite struggling for fitness and form in his valedictory season, Bolt had still been favourite to secure his 20th global gold.
It was supposed to be Gatlin's year in 2015, when the American went to the World Championships in Beijing on the back of a 28-race unbeaten run.
Bolt produced his great miracle to beat him that night in the Bird's Nest, but in the stadium where he won 100m Olympic gold in 2012, he could not provide the perfect ending to a perfect career.